A single channel pipette is an essential tool for laboratory work and can be used to accurately measure, transfer, and mix volumes of liquid. Calibrating a pipette properly is crucial for ensuring accurate results.
This article will provide step-by-step instructions on how to properly calibrate single channel pipettes with seven easy methods that anyone can follow.
- Use Proper Technique
When calibrating a pipette, make sure to hold the pipette in the correct way and keep your finger off of the plunger during the calibration process. This will help ensure that you get accurate readings and won’t damage any components.
- Check for Leaks
Make sure to check for leaks by putting the pipette in a water bath and then pulling the plunger back. If you see any bubbles coming from the tip of your pipette, it means that there is a leak present.
- Check the Temperature
A well-calibrated pipette relies on accurate temperature readings. Before you start your calibration procedure, it’s important to check that the temperature of your work area is between 16 and 25 degrees Celsius.
If your work environment is too hot or cold, this can affect the accuracy of your results. To ensure you’re working in optimal conditions, use a thermometer to check the temperature and adjust your workspace accordingly.
- Set Up Your Pipette
Once you have established an ideal temperature for your work area, it’s time to set up your pipette. Make sure all of the components are securely attached – this includes the tip, the ejector button, and any other parts that may be included. Once everything is properly attached, you’re ready to begin the calibration process.
- Calibrate the Volume
The next step is single channel pipettes the volume of your pipette. To do this, fill a container with distilled water and use your pipette to measure out a set amount. This number should be specified in the instructions included with your pipette.
Make sure you are measuring accurately and that there is no air bubble present in the container before taking a reading. Once you have taken your measurement, compare it to manufacturer’s specifications and adjust accordingly.
- Test the Delivery
After calibrating the volume, it’s time to test the delivery. To do this, fill a container with distilled water and use your pipette to deliver liquid into another container. Measure the amount of liquid delivered to make sure it matches what you calibrated in step five.
If there is a discrepancy, adjust accordingly until you have achieved the desired level of accuracy.
- Record Your Results
The last step in calibrating a single channel pipette is to record your results. Make sure you write down any adjustments that you made so that you can refer back to them in the future, if necessary. This will help you ensure that your pipettes are always functioning accurately.
It is also a good idea to keep your records in a safe place, such as a lab notebook or logbook.
Following these steps will help you calibrate your single channel pipette with accuracy and confidence. Always remember to check the temperature of your work area before beginning any calibration process and make sure all pipette components are securely attached.
Taking the time to properly calibrate your pipette will help you achieve accurate results each and every time.